MersV1, Main, Exploration, bibRecord, 002946

Structural basis for recognition of Co2+ by RNA aptamers.

Identifieur interne : 002946 ( Main/Exploration ); précédent : 002945; suivant : 002947

Structural basis for recognition of Co2+ by RNA aptamers.

Auteurs : Jan Wrzesinski [Pologne] ; Stanisław K. J Wiakowski

Source :

The FEBS journal [ 1742-464X ] ; 2008.

RBID : pubmed:18312410

Descripteurs français

KwdFr :
- Aptamères nucléotidiques (), Aptamères nucléotidiques (synthèse chimique), Cations divalents (), Cobalt (), Conformation d'acide nucléique, Données de séquences moléculaires, Phosphates (), Purines (), Sites de fixation, Solutions, Séquence nucléotidique, Thionucléosides ().
MESH :
- synthèse chimique : Aptamères nucléotidiques.
- Aptamères nucléotidiques, Cations divalents, Cobalt, Conformation d'acide nucléique, Données de séquences moléculaires, Phosphates, Purines, Sites de fixation, Solutions, Séquence nucléotidique, Thionucléosides.

English descriptors

KwdEn :
- Aptamers, Nucleotide (chemical synthesis), Aptamers, Nucleotide (chemistry), Base Sequence, Binding Sites, Cations, Divalent (chemistry), Cobalt (chemistry), Molecular Sequence Data, Nucleic Acid Conformation, Phosphates (chemistry), Purines (chemistry), Solutions, Thionucleosides (chemistry).
MESH :
- chemical , chemical synthesis : Aptamers, Nucleotide.
- chemical , chemistry : Aptamers, Nucleotide, Cations, Divalent, Cobalt, Phosphates, Purines, Thionucleosides.
- Base Sequence, Binding Sites, Molecular Sequence Data, Nucleic Acid Conformation, Solutions.

Abstract

Co(2+) binding RNA aptamers were chosen as research models to reveal the structural basis underlying the recognition of Co(2+) by RNA, with the application of two distinct methods. Using the nucleotide analog interference mapping assay, we found strong interference effects after incorporation of the 7-deaza guanosine phosphorotioate analog into the RNA chain at equivalent positions G27 and G28 in aptamer no. 18 and G25 and G26 in aptamer no. 20. The results obtained by nucleotide analog interference mapping suggest that these guanine bases are crucial for the creation of Co(2+) binding sites and that they appear to be involved in the coordination of the ion to the exposed N7 atom of the tandem guanines. Additionally, most 7-deaza guanosine phosphorotioate and 7-deaza adenosine phosphorotioate interferences were located in the common motifs: loop E-like in aptamer no. 18 and kissing dimer in aptamer no. 20. We also found that purine-rich stretches containing guanines with the highest interference values were the targets for hybridization of 6-mers, which are members of the semi-random oligodeoxyribonucleotide library in both aptamers. It transpired that DNA oligomer directed RNase H digestions are sensitive to Co(2+) and, at an elevated metal ion concentration, the hybridization of oligomers to aptamer targets is inhibited, probably due to higher stability and complexity of the RNA structure.

DOI: 10.1111/j.1742-4658.2008.06320.x
PubMed: 18312410

Affiliations:

Pologne

Le document en format XML

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<term>Cations, Divalent (chemistry)</term>
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<term>Conformation d'acide nucléique</term>
<term>Données de séquences moléculaires</term>
<term>Phosphates ()</term>
<term>Purines ()</term>
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<term>Solutions</term>
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<term>Phosphates</term>
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<term>Binding Sites</term>
<term>Molecular Sequence Data</term>
<term>Nucleic Acid Conformation</term>
<term>Solutions</term>
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<term>Phosphates</term>
<term>Purines</term>
<term>Sites de fixation</term>
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<front><div type="abstract" xml:lang="en">Co(2+) binding RNA aptamers were chosen as research models to reveal the structural basis underlying the recognition of Co(2+) by RNA, with the application of two distinct methods. Using the nucleotide analog interference mapping assay, we found strong interference effects after incorporation of the 7-deaza guanosine phosphorotioate analog into the RNA chain at equivalent positions G27 and G28 in aptamer no. 18 and G25 and G26 in aptamer no. 20. The results obtained by nucleotide analog interference mapping suggest that these guanine bases are crucial for the creation of Co(2+) binding sites and that they appear to be involved in the coordination of the ion to the exposed N7 atom of the tandem guanines. Additionally, most 7-deaza guanosine phosphorotioate and 7-deaza adenosine phosphorotioate interferences were located in the common motifs: loop E-like in aptamer no. 18 and kissing dimer in aptamer no. 20. We also found that purine-rich stretches containing guanines with the highest interference values were the targets for hybridization of 6-mers, which are members of the semi-random oligodeoxyribonucleotide library in both aptamers. It transpired that DNA oligomer directed RNase H digestions are sensitive to Co(2+) and, at an elevated metal ion concentration, the hybridization of oligomers to aptamer targets is inhibited, probably due to higher stability and complexity of the RNA structure.</div>
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Serveur d'exploration MERS

Structural basis for recognition of Co2+ by RNA aptamers.

Structural basis for recognition of Co2+ by RNA aptamers.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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